メンバー紹介
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教授
水島 昇
プロフィール
1985年 武蔵高等学校卒業 1991年 東京医科歯科大学医学部医学科卒業 1996年 同大学院医学研究科修了 博士（医学） 1997年 岡崎国立共同研究機構基礎生物学研究所 ポスドク・さきがけ研究員・助手など 2004年 (財)東京都医学研究機構 東京都臨床医学総合研究所 室長 2006年 東京医科歯科大学医学部・大学院医歯学総合研究科 細胞生理学分野 教授 2012年 東京大学医学部・大学院医学系研究科 分子生物学分野 教授（現在に至る）
主要論文
詳しくは 「発表論文/Selected publications」
受賞
お茶の水会医科同窓会奨励賞（1999年）、日本生化学会奨励賞（2001年）、日本分子生物学会三菱化学奨励賞（2005年）、文部科学大臣表彰若手科学者賞（2006年）、FEBS Letters Young Scientist Award（2007年）、日本学術振興会賞（2008年）、塚原仲晃記念賞（2008年）、井上学術賞（2009年）、日本生化学会柿内三郎記念賞（2010年）、武田医学賞（2011年）、トムソン・ロイター引用栄誉賞（2013年）、読売テクノフォーラム・ゴールドメダル賞（2014年）、永瀬特別賞（2014年）、抗加齢医学会学会賞（2015年）、上原賞（2016年）、持田記念学術賞（2016年）、高峰記念第一三共賞（2017年）、藤原賞（2020年）、日本医師会医学賞（2020年）、日本医療研究開発大賞文部科学大臣賞（2020年）、紫綬褒章（2021年）、Beth Levine, M.D. Prize in Autophagy Research（2023年）、2014～2023年 Clarivate Analytics社 Highly Cited Researchers（10年連続）
学会
日本生化学会（副会長・元会長）、日本分子生物学会（副理事長）、日本細胞生物学会（理事）、The American Society for Cell Biology、The American Society for Clinical Investigation (Membership)、EMBO(Associate membership)
雑誌編集
eLife (reviewing editor)、Molecular Cell (editorial board)、Autophagy (associate editor)、Cell Structure and Function (associate editor)、Genes to Cells (associate editor)、Journal of Biochemistry (advisory board)
原著論文
Nagashima, Y., *Eguchi, T., Koyama-Honda, I., *Mizushima, N. Optogenetic tools for inducing organelle membrane rupture. bioRxiv doi: 10.1101/2024.08.13.607738 Hama, Y., Kurikawa, Y., Matsui, T., *Mizushima, N., *Yamamoto, H. TAX1BP1 recruits ATG9 vesicles through SCAMP3 binding. bioRxiv 2023.08.18.553817; doi: https://doi.org/10.1101/2023.08.18.553817 Yang, Z., Yoshii, S.R., Sakai, Y., Chino, H., Knorr, R.L. *Mizushima, N. Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43:5613-5634 (2024). doi.org/10.1038/s44318-024-00272-5 Kurikawa, Y., *Koyama-Honda, I., Igarashi, K., Tamura, N., Koike, S., *Mizushima, N. Organelle landscape analysis using a multi-parametric particle-based method. PLOS Biol 22: e3002777 (2024). doi: 10.1371/journal.pbio.3002777 Shinoda, S., Sakai, Y., Matsui, T., Uematsu, M., Koyama-Honda, I., Sakamaki, J.I., Yamamoto, H., *Mizushima, N. Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation. eLife 12:RP92189 (2024). doi: 10.7554/eLife.92189. Eguchi, T., Sakurai, M., Wang, Y., Saito, C., Yoshii, G., Wileman, T., Mizushima, N., *Kuwahara, T., *Iwatsubo, T. The V-ATPase-ATG16L1 axis recruits LRRK2 to facilitate the lysosomal stress response. J. Cell Biol., 223:e202302067 (2024) *Sakai, Y., Takahashi, S., Koyama-Honda, I., Saito, C., *Mizushima, N. Experimental determination and mathematical modeling of standard shapes of forming autophagosomes. Nat Commun 15:91 (2024) doi: 10.1038/s41467-023-44442-1 Schuermans, N., El Chehadeh, S., Hemelsoet, D., Gautheron, J., Vantyghem, M.C., Nouioua, S., Tazir, M., Vigouroux, C., Auclair, M., Bogaert, E., Dufour, S., Okawa, F., Hilbert, P., Van Doninck, N., Taquet, M.C., Rosseel, T., De Clercq, G., Debackere, E., Van Haverbeke, C., Cherif, F.R., Urtizberea, J.A., Chanson, J.B., Funalot, B., Authier, F.J., Kaya, S., Terryn, W., Callens, S., Depypere, B., Van Dorpe, J. Program for Undiagnosed Diseases (UD-PrOZA), Poppe, B., Impens, F., Mizushima, N., Depienne, C., Jéru, I., Dermaut, B. Loss of phospholipase PLAAT3 causes a mixed lipodystrophic and neurological syndrome due to impaired PPAR𝛾 signaling. Nat. Genet. doi: 10.1038/s41588-023-01535-3. Online ahead of print. Shimizu, T., Tamura, N., Nishimura, T., Saito, C., Yamamoto, H., *Mizushima, N., Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration. Hum Mol Genet. 32: 2623-2637 (2023).doi: 10.1093/hmg/ddad096. *Nishimura, T., Lazzeri, G., Mizushima, N., *Covino, R., Tooze, S. Unique Amphipathic α-helix Drives Membrane Insertion and Enzymatic Activity of ATG3. Sci Adv 9: eadh1281 (2023) Ishii, S., Chinob, H. Ode, K.L., Kurikawa, Y., Ueda, H.R., Matsuura, A., *Mizushima, N., *Itakura, E. CCPG1 recognizes ER luminal proteins for selective ER-phagy. Mol Biol Cell. 34:ar29 (2023) doi: 10.1091/mbc.E22-09-0432 Shimasawa, M., Sakamaki, J.I., *Mizushima, N. The pH-sensing Rim101 pathway regulates cell size in budding yeast. J. Biol. Chem. 299: 102973 (2023) Fu, J., Zhao, L., Pang, Y., Chen, H., Yamamoto, H., Chen, Y., Li, Z., Mizushima, N., *Jia H. Apicoplast biogenesis mediated by ATG8 requires the ATG12–-ATG5-ATG16L and SNAP29 complexes in Toxoplasma gondii. Autophagy 19:1258-1276 (2023) Takahashi S, Saito C, *Koyama-Honda I, *Mizushima, N. Quantitative 3D correlative light and electron microscopy of organelle association during autophagy. Cell Struct Funct. 47:89-99 (2022) doi: 10.1247/csf.22071 Yim, W.W., *Yamamoto, H., *Mizushima, N. A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. eLife 11:e78923 (2022) doi: 10.7554/eLife.78923 Ohshima, T., Yamamoto, H., Sakamaki, Y., Saito, C., *Mizushima, N. NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and endosomal microferritinophagy. J Cell Biol. 221:e202203102 (2022) doi: 10.1083/jcb.202203102 Sakamaki, J.I., Ode, K.L., Kurikawa,Y., Ueda, H.R., Yamamoto, H., *Mizushima, N. Ubiquitination of phosphatidylethanolamine in organellar membranes. Mol. Cell 82:3677-3692 (2022) doi: 10.1016/j.molcel.2022.08.008 Chino, H., Yamasaki, A., Ode, K.L., Ueda, H.R., *Noda, N.N., *Mizushima, N. Phosphorylation by casein kinase 2 ensures ER-phagy receptor TEX264 binding to ATG8 proteins. EMBO Rep. 23:e54801 (2022). Zhang, S., Yazaki, E., Sakamoto, H., Yamamoto, H., *Mizushima, N. Evolutionary diversification of the autophagy-related ubiquitin-like conjugation systems. Autophagy 18:2969-2984 (2022). Yim, W.W., Yamamoto, H., *Mizushima, N. Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair. FEBS Lett. 596:991-1003 (2022) Yim, W.W., Kurikawa, Y., *Mizushima, N. An exploratory text analysis of the autophagy research field. Autophagy 18:1648-1661 (2022) Kusumaatmaja, H., May, A.I., Feeney, M., McKenna, J.F., Mizushima, N., Frigerio, L., *Knorr, R.L. Wetting of phase-separated droplets on plant vacuole membranes leads to a competition between tonoplast budding and nanotube formation. Proc. Natl. Acad. Sci. USA. 118: e2024109118 (2021) Yamamoto, Y., Chino, H., Tsukamoto, S., Ode, K. L., Ueda, H. R., Mizushima, N. NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA. Nat Commun 12(1):3292 (2021). Mimura, K., Sakamaki, J.I., Morishita, H., Kawazu, M., Mano, H., *Mizushima, N. Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy J. Biol. Chem. 296:100780 (2021) *Morishita, H., Eguchi, T., Tsukamoto, S., Sakamaki, Y., Takahashi, S., Saito, C., Koyama-Honda, I., *Mizushima, N. Organelle degradation in the lens by PLAAT phospholipases. Nature, 592:634-638 (2021) Okawa, F., Hama, Y., Zhang, S., Morishita, H., Yamamoto, H., Levine, T.P., *Mizushima, N. Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1. J. Cell Sci. 134:jcs255877 (2021) Agudo-Canalejo, J., Schultz, S.W., Chino, H., Migliano, S., Saito, C., Koyama-Honda, I., Stenmark, H., Brech, A., May, A.I., Mizushima, N., *Knorr, R. L. Wetting regulates autophagy of phase separated droplets and the cytosol. Nature 591:142-146 (2021) Morishita, H., Kanda, Y., Kaizuka, T., Chino, H., Nakao, K., Miki, Y., Taketomi, Y., Guan J.L., Murakami, M., Aiba, A., *Mizushima, N. Autophagy is required for maturation of surfactant-containing lamellar bodies in the lung and swim bladder. Cell Rep. 33:108477 (2020) Maeda, S., Yamamoto, H., Kinch, L.N., Garza, C.M., Takahashi, S., Otomo, C., Grishin, N.V., Forli, S., Mizushima, N., and *Otomo, T. Structure, lipid scrambling activity and role in autophagosome formation of ATG9A. Nat. Struct. Mol. Biol. 27:1194-1201 (2020) *Sakai, Y., Koyama-Honda, I., Tachikawa, M., Knorr, R.L., *Mizushima, N. Modeling membrane morphological change during autophagosome formation. iScience 23, 9, 101466 (2020) Takahashi, S.S., Sou, Y.S., Saito, T., Kuma, A., Yabe, T., Sugiura, Y., Lee, H.C., Suematsu, M., Yokomizo, T., Koike, M., Terai, S., Mizushima, N., Waguri, S., Komatsu, M. Loss of autophagy impairs physiological steatosis by accumulation of NCoR1. Life Sci. Alliance 3. pii: e201900513 (2019) Morishita, H., Zhao, Y.G., Tamura, N., Nishimura, T., Kanda, Y., Sakamaki, Y., Okazaki, M., Li, D., *Mizushima, N. A critical role of VMP1 in lipoprotein secretion. Elife 8. pii: e48834 (2019) Chino, H., Hatta, T., Natsume, T., *Mizushima, N. Intrinsically disordered protein TEX264 mediates ER-phagy. Mol. Cell 74:909-921 (2019) DeVorkin, L., Pavey, N., Carleton, G., Comber, A., Ho, C., Lim, J., McNamara, E., Huang, H., Kim, P., Zacharias, L.G., Mizushima, N., Saitoh, T., Akira, S., Beckham, W., Lorzadeh, A., Moksa, M., Cao, Q., Murthy, A., Hirst, M., DeBerardinis, R.J., *Lum, J.J. Autophagy Regulation of Metabolism Is Required for CD8+ T Cell Anti-tumor Immunity. Cell Rep. 27:502-513 (2019) Saito, T., Kuma, A., Sugiura, Y., Ichimura, Y., Obata, M., Kitamura, H., Okuda, S., Lee, H.C., Ikeda, K., Kanegae, Y., Saito, I., Auwerx, J., Motohashi, H., Suematsu, M., Soga, T., Yokomizo, T., Waguri, S., Mizushima, N., *Komatsu, M. Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nat Commun. 10:1567 (2019) Pang, Y., Yamamoto, H., Sakamoto, H., Oku, M., Mutungi, J.K., Sahani, M.H, Kurikawa, Y., Kita, K., Noda, N.N., Sakai, Y., *Jia, H., *Mizushima, N. Evolution from covalent conjugation to non-covalent interaction in the ubiquitin-like ATG12 system. Nat. Struct. Mol. Biol. 26:289-296 (2019) Morita, K., Hama, Y., Izume, T., Tamura, N., Ueno, T., Yamashita, Y., Sakamaki, Y., Mimura, K., Morishita, H., Shihoya, W., Nureki, O., Mano, H., *Mizushima, N. Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation. J Cell Biol. 217:3817-3828 (2018) Matsui, T., Jiang, P., Nakano, S., Sakamaki, Y., Yamamoto, H., *Mizushima, N. Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17. J Cell Biol. 217:2633-2645 (2018) Takahashi, S., Kagami, Y., Hanaoka, K., Terai, T., Komatsu, T., Ueno, T., Uchiyama, M., Koyama-Honda, I., Mizushima, N., Taguchi, T., Arai, H., Nagano, T., Urano, Y. Development of a series of practical fluorescent chemical tools to measure pH values in living samples. J Am Chem Soc. 140:5925-5933 (2018) Wallot-Hieke, N., Verma, N., Schlütermann, D., Berleth, N., Deitersen, J., Böhler, P., Stuhldreier, F., Wu, W., Seggewiß, S., Peter, C., Gohlke, H., Mizushima, N., *Stork, B. Systematic analysis of ATG13 domain requirements for autophagy induction. Autophagy. 14:743-763 (2018) Tamura, N., Nishimura, T., Sakamaki, Y., Koyama-Honda, I., Yamamoto, H., *Mizushima, N. Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591:3819-3830 (2017) Fujimoto, C., Iwasaki, S., Urata, S., Morishita, H., Sakamaki, Y., Fujioka, M., Kondo, K., Mizushima, N., Yamasoba, T. Autophagy is essential for hearing in mice. Cell Death Dis. 8(5):e2780 (2017) Nishimura, T., Tamura, N., Kono, N., Shimanaka, Y., Arai, H., Yamamoto, H., *Mizushima, N. Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains. EMBO J. 36:1719-1735 (2017) Uematsu, M., Nishimura, T., Sakamaki, Y., Yamamoto, H., *Mizushima, N. Accumulation of undegraded autophagosomes by expression of dominant-negative syntaxin 17 mutants. Autophagy 9:1-13 (2017) Kimura, H., Eguchi, S., Sasaki, J., Kuba, K., Nakanishi, H., Takasuga, S., Yamazaki, M., Goto, A., Watanabe, H., Itoh, H., Imai, Y., Suzuki, A., Mizushima, N., *Sasaki, T. Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy. JCI Insight 2(1):e89462 (2017) Fujita, N., Huang, W., Lin, T.H., Groulx, J.F., Jean, S., Kuchitsu, Y., Koyama-Honda, I., Mizushima, N., Fukuda, M., *Kiger, A.A. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife 10.7554/eLife.23367 (2017) Tsuboyama, K., Koyama-Honda, I., Sakamaki, Y., Koike, M., Morishita, H., *Mizushima, N. The ATG conjugation systems are important for degradation of the inner autophagosomal membrane. Science 354:1036-1041 (2016) Kaizuka, T., Morishita, H., Hama, Y., Tsukamoto, S., Matsui, T., Toyota, Y., Kodama, A., Ishihara, T., Mizushima, T., *Mizushima, N. An autophagic flux probe that releases an internal control. Mol. Cell 64: 835-849 (2016) Yoshii, S.R., Kuma, A., Akashi, T., Hara, T., Yamamoto, A., Kurikawa, Y., Itakura, E., Tsukamoto, S., Shitara, H., Eishi, Y., *Mizushima, N. Systemic analysis of Atg5-null mice rescued from neonatal lethality by transgenic ATG5 expression in neurons. Dev. Cell 39: 116-130 (2016) Wang, C., Chen, S., Yeo, S., Karsli-Uzunbas, G., White, E., Mizushima, N., Virgin, H.W., Guan, J.L. Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide. J. Cell Biol. 212: 545-60 (2016) Kaizuka, T., *Mizushima, N. Atg13 is essential for autophagy and cardiac development in mice. Mol. Cell. Biol. 36: 585-595 (2015) Hieke, N., Löffler, A.S., Kaizuka, T., Berleth, N., Böhler, P., Drießen, S., Stuhldreier, F., Friesen, O., Assani, K., Schmitz, K., Peter, C., Diedrich, B., Dengjel, J., Holland, P., Simonsen, A., Wesselborg, S., Mizushima, N., *Stork, B. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy. 11(9):1471-83 (2015) Suzuki, H., Kaizuka, T., *Mizushima, N., *Noda, N. N. Structure of the Atg101–Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Nat. Struct. Mol. Biol. 22: 572–580 (2015) Zhao, Y.G., Sun, L., Miao, G., Ji, C., Zhao, H., Sun, H., Miao, L., Yoshii, S.R., Mizushima, N., Wang, X., Zhang, H. The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis. Autophagy. 11:881-90 (2015) Schlie, K., Westerback, A., DeVorkin, L., Hughson, L.R., Brandon, J.M., MacPherson, S., Gadawski, I., Townsend, K.N., Poon, V.I., Elrick, M.A., Côté, H.C., Abraham, N., Wherry, E.J., Mizushima, N., Lum, J.J. Survival of Effector CD8+ T Cells during Influenza Infection Is Dependent on Autophagy. J Immunol. 194: 4277-4286 (2015) Ishihara, T., Ban-Ishihara, R., Maeda, M., Matsunaga, Y., Ichimura, A., Kyogoku, S., Aoki, H., Katada, S., Nakada, K., Nomura, M., Mizushima, N., Mihara, K., Ishihara, N. Dynamics of Mitochondrial DNA Nucleoids Regulated by Mitochondrial Fission Is Essential for Maintenance of Homogeneously Active Mitochondria during Neonatal Heart Development. Mol Cell Biol. 35:211-23 (2015) Lin, H.H., Lin, S.M., Chung, Y., Vonderfecht, S., Camden, J.M., Flodby, P., Borok, Z., Limesand, K.H., Mizushima, N., Ann, D.K. Dynamic involvement of ATG5 in cellular stress responses. Cell Death Dis. 5:e1478 (2014) Udagawa, O., Ishihara, T., Maeda, M., Matsunaga, Y., Tsukamoto, S., Kawano, N., Miyado, K., Shitara, H., Yokota, S., Nomura, M., Mihara, K., Mizushima, N., Ishihara, N. Mitochondrial Fission Factor Drp1 Maintains Oocyte Quality via Dynamic Rearrangement of Multiple Organelles. Curr. Biol. 24:2451-8 (2014) Kishi-Itakura, C, Koyama-Honda, I., Itakura, E., Mizushima, N. Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells. J. Cell Sci. 127:4089-4102 (2014) Ogasawara, Y., Itakura, E., Kono, N., Mizushima, N., Arai, H., Nara, A., Mizukami, T., Yamamoto, A. Stearoyl-CoA Desaturase 1 Activity Is Required for Autophagosome Formation. J. Biol. Chem. 289:23938-50 (2014) Yamamoto, A., Mizushima, N., Tsukamoto, S. Fertilization-Induced Autophagy in Mouse Embryos Is Independent of mTORC1. Biol. Reprod. 91:7, 1–7 (2014) Jiang, P., Nishimura, T., Sakamaki, Y., Itakura, E., Hatta, T., Natsume, T., Mizushima, N. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell. 25:1327-1337 (2014) Watanabe-Asano, T., Kuma, A., Mizushima N. Cycloheximide inhibits starvation-induced autophagy through mTORC1 activation. Biochem. Biophys. Res. Commun. 445:334-339 (2014) Sahani, M.H., Itakura, E., Mizushima, N. Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids. Autophagy. 10:431-441 (2014) Karanasios, E., Stapleton, E., Manifava, M., Kaizuka, T., Mizushima, N., Walker, S.A., Ktistakis, N.T. Dynamic association of the ULK1 complex with omegasomes during autophagy induction. J. Cell Sci. 126:5224-38 (2013) Koyama-Honda, I., Itakura, E., Fujiwara, T.K., Mizushima, N. Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9:1491-1499 (2013) Seino, J., Wang, L., Harada, Y., Huang, C., Ishii, K., Mizushima, N., Suzuki, T. Basal autophagy is required for the efficient catabolism of sialyloligosaccharides. J. Biol. Chem. 288:26898-26907 (2013) Naito, T., Kuma, A., Mizushima, N. Differential contribution of insulin and amino acids to the mTORC1-autophagy pathway in the liver and muscle. J. Biol. Chem. 288:21074-21081 (2013) Morishita, H., Eguchi, S., Kimura, H., Sasaki, J., Sakamaki, Y., Robinson, M.L., Sasaki, T., Mizushima, N. Deletion of autophagy-related 5 (Atg5) and Pik3c3 in the lens causes cataract independent of programmed organelle degradation. J. Biol. Chem. 288:11436-11447 (2013) Saitsu, H., Nishimura, T., Muramatsu, K., Kodera, H., Kumada, S., Sugai, K., Kasai-Yoshida, E., Sawaura, N., Nishida, H., Hoshino, A., Ryujin, F., Yoshioka, S., Nishiyama, K., Kondo, Y., Tsurusaki, Y., Nakashima, M., Miyake, N., Arakawa, H., Kato, M., Mizushima, N., Matsumoto, N. De novo mutations in the autophagy gene encoding WDR45 (WIPI4) cause static encephalopathy of childhood with neurodegeneration in adulthood. Nat. Genet. 45: 445-449 (2013) Nishimura, T., Kaizuka, T., Cadwell, K., Sahani, M.H., Saitoh, T., Akira, S., Virgin, H.W. Mizushima, N. FIP200 regulates targeting of Atg16L1 to the isolation membrane. EMBO Rep. 14: 284-291 (2013) Quy, P.N., Kuma, A., Pierre, P., Mizushima, N. Proteasome-dependent activation of mammalian target of rapamycin complex 1 (mTORC1) is essential for autophagy suppression and muscle remodeling following denervation. J. Biol. Chem. 288: 1125-1134 (2013) Itakura, E., Kishi-Itakura, C., Mizushima, N. The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151: 1256-1269 (2012) Onoue, K., Jofuku, A., Ban-Ishihara, R., Ishihara, T., Maeda, M., Koshiba, T., Itoh, T., Fukuda, M., Otera, H., Oka, T., Takano, H., Mizushima, N., Mihara, K., Ishihara, N. Fis1 acts as mitochondrial recruitment factor for TBC1D15 that involved in regulation of mitochondrial morphology. J. Cell Sci. 126: 176-185 (2012) Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizuno Y, Mizushima N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H. Mitochondrial dysfunction associated with increased oxidative stress and alpha-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue. Mol. Brain. 5:35 (2012) Lin, T.C., Chen, Y.R., Kensicki, E., Li, A.Y., Kong, M., Li, Y., Mohney, R.P., Shen, H.M., Stiles, B., Mizushima, N., Lin, L.I., Ann, D.K. Autophagy: Resetting glutamine-dependent metabolism and oxygen consumption. Autophagy 8: 1477-1493 (2012) Kitamura, K., Kishi-Itakura, C., Tsuboi, T., Sato, S., Kita, K., Ohta, N., Mizushima, N. Autophagy-Related Atg8 Localizes to the Apicoplast of the Human Malaria Parasite Plasmodium falciparum. PLoS One 7:e42977 (2012) Liu, S., Hartleben, B., Kretz, O., Wiech, T., Igarashi, P., Mizushima, N., Walz, G., Huber, T.B. Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury. Autophagy 8: 826-837 (2012) Itakura, E., Kishi-Itakura, C., Koyama-Honda, I., Mizushima, N. Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy. J. Cell Sci. 125: 1488-1499 (2012) Velikkakath, A.K.G, Nishimura, T., Oita, E., Ishihara, N., Mizushima, N. Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol. Biol. Cell 23: 896-909 (2012) Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18:1042-1052 (2011) Yoshii, S.R., Kishi, C., Ishihara, N., Mizushima, N. Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286:19630-19640 (2011) Asano, T., Komatsu, M., Yamaguchi-Iwai, Y., Ishikawa, F., Mizushima, N., Iwai, K. Distinct mechanisms of ferritin delivery to lysosomes in iron depleted and iron-replete cells. Mol. 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The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells Mol. Biol. Cell 11, 747-63 (2000) George, M. D., Baba, M., Scott, S. V., Mizushima, N., Garrison, B. S., Ohsumi, Y. and Klionsky, D. J. Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways [In Process Citation] Mol. Biol. Cell 11, 969-82 (2000) Shintani, T., Mizushima, N., Ogawa, Y., Matsuura, A., Noda, T. and Ohsumi, Y. Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast EMBO J. 18, 5234-5241 (1999) Tanida, I., Mizushima, N., Kiyooka, M., Ohsumi, M., Ueno, T., Ohsumi, Y. and Kominami, E. Apg7p/Cvt2p: a novel protein activating enzyme essential for autophagy Mol. Biol. Cell 10, 1367-1379 (1999) Takenaga, M., Igarashi, R., Matsumoto, K., Takeuchi, J., Mizushima, N., Nakayama, T., Morizawa, Y. and Mizushima, Y. 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Dis. 57, 495-499 (1998) Mizushima, N., Kohsaka, H., Nanki, T., Ollier, W. E. R., Carson, D. A. and Miyasaka, N. HLA dependent peripheral T cell receptor repertoire formation and its modification by rheumatoid arthritis Clin. Exp. Immunol. 110, 428-433 (1997) Nanki, T., Kohsaka, H., Mizushima, N., Ollier, W. E. R., Carson, D. A. and Miyasaka, N. Genetic control of T cell receptor BJ gene expression in peripheral lymphocytes of normal and rheumatoid arthritis monozygotic twins J. Clin. Invest. 98, 1594-1601 (1996) Mizushima, N., Koike, R., Kohsaka, H., Kushi, Y., Handa, S., Yagita, H. and Miyasaka, N. Ceramide induces apoptosis via CPP32 activation FEBS Lett. 395, 267-271 (1996) Hayashi, H., Mizushima, N., Yoshinaga, H., Kawamitsu, H., Matsuda, S., Tanoue, M., Kurosawa, H., Fujimoto, H. and Amakawa, T. The relationship between lipoprotein(a) and low density lipoprotein receptors during the treatment of hyperthyroidism Horm. Metab.Res. 28, 384-387 (1996) Mizushima, N., Kohsaka, H., Tsubota, K., Saito, I. and Miyasaka, N. Diverse T cell receptor beta gene usage by infiltrating T cells in the lacrimal glands of Sjogren's syndrome Clin. Exp. Immunol. 101, 33-38 (1995) 総説論文
*Mizushima, N. Ubiquitin in autophagy and non-protein ubiquitination. Nat. Struct. Mol. Biol. 31:208-209 (2024) doi: 10.1038/s41594-024-01217 *Sakamaki, J.-i., *Mizushima, N. Ubiquitination of non-protein substrates. Trends Cell Biol. 33: 991-1003 (2023) doi: 10.1016/j.tcb.2023.03.014 *Sakamaki, J.-i., *Mizushima, N. Cell biology of protein–lipid conjugation. Cell Struct. Funct. 48: 99-112 (2023) doi: 10.1247/csf.23016 Liton, P.B., Boesze-Battaglia, K., Boulton, M.E., Boya, P., Ferguson, T.A., Ganley, I.G., Kauppinnen, A., Laurie, G.W., *Mizushima, N., Morishita, H., Russok, R., Sadda, J., Shyam, R., Sinha, D., Thompson, D.A., and Zacks. D.N. Autophagy in the eye: from physiology to pathophysiology. Autophagy Rep. 1: 2178996 (2023) doi.org/10.1080/27694127.2023.2178996 Yamamoto, H., Zhang, S., *Mizushima, N. Autophagy genes in biology and disease. Nat. Rev. Genet. 24:382-400 (2023) doi: 10.1038/s41576-022-00562-w *Sakamaki, J.-i., *Mizushima, N. Protocols to detect ubiquitinated phospholipids by immunoblotting. STAR Protoc. 4:101935 (2022) doi: 10.1016/j.xpro.2022.101935 Holthuis, J.C.M., Jahn, H., Menon, A.K., Mizushima, N. An alliance between lipid transfer proteins and scramblases for membrane expansion. Fac. Rev. 11:22 (2022) *Mizushima, N. SnapShot: Organelle degradation. Mol. Cell 82:1604-1604.e1 (2022) Chino, H., *Mizushima, N. ER-phagy: quality and quantity control of the endoplasmic reticulum by autophagy. Cold Spring Harb. Perspect. Biol. 8:a041256 (2022) Hama, Y., Morishita, H., *Mizushima, N. Regulation of ER-derived membrane dynamics by the DedA domain-containing proteins VMP1 and TMEM41B. 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Breakthroughs and bottlenecks in autophagy research. Trends Mol. Med. 11;S1471-4914(21)00179-9 (2021) Yamamoto, Y., *Mizushima, N. Autophagy and ciliogenesis. JMA J. 15:207-215 (2021) Klionsky et al. "Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)." Autophagy 17:1-382 (2021) Morishita, H., Kanda, Y., *Mizushima, N. No air without autophagy: autophagy is important for lung and swim bladder inflation. Autophagy, 17(4):1040-1041 (2021) Schultz, S.W., Agudo-Canalejo, J., Chino, H., Migliano, S., Saito, C., Koyama-Honda, I., Stenmark, H., Brech, A., Mizushima, N., *Knorr, R. L., *May, A.I. Should I bend or should I grow: the mechanisms of droplet-mediated autophagosome formation. Autophagy, 17:1046-1048 (2021) Yim, W.W., *Mizushima, N. Autophagosome maturation stymied by SARS-CoV-2. Dev. Cell 56:400-402 (2021) *Mizushima, N., Levine, B. Autophagy in human diseases. N. Engl. J. Med 383:1564-1576 (2020) *Simon, A.K., *Mizushima, N. Beth Levine 1960-2020. Nat. Cell Biol. 22:909-910 (2020) Mizushima et al., Autophagy assays for biological discovery and therapeutic development, Trends Biochem Sci. (2020) Chino, H., *Mizushima, N. ER-phagy: Quality control and turnover of endoplasmic reticulum. Trends Cell Biol. 30:384-398 (2020) Yim, W.W., *Mizushima, N. Lysosome biology in autophagy. Cell Discov. 6:6 (2020) Mizushima, N. The ubiquitin E2 enzyme UBE2QL1 mediates lysophagy. EMBO Rep. 15:e49104 (2019) Morishita, H., *Mizushima, N. Diverse cellular roles of autophagy. Annu. Rev. Cell Dev. Biol. 35:453-475 (2019) Morita, K., Hama, Y., *Mizushima, N. TMEM41B functions with VMP1 in autophagosome formation. Autophagy. 15:922-923 (2019) Mizushima, N., Matsui, T., Yamamoto, H. YKT6 as a second SNARE protein of mammalian autophagosomes. Autophagy. 15:176-177 (2019) Deretic, V. et al. Autophagy, Inflammation, and Metabolism (AIM) Center of Biomedical Research Excellence: supporting the next generation of autophagy researchers and fostering international collaborations. Autophagy. 14:925-929 (2018) Mizushima, N. A brief history of autophagy from cell biology to physiology and disease. Nat. Cell Biol. 20:521-527 (2018) Mizushima, N. A dual binding receptor for ER-phagy. Dev. Cell 44:133-135 (2018) Yoshii, S.R., Mizushima, N. Monitoring and measuring autophagy. Int. J. Mol. Sci. 18(9). pii: E1865 (2017) Kuma, A., Komatsu, M., Mizushima, N. Autophagy-monitoring and autophagy-deficient mice. Autophagy. 13:1619-1628 (2017) Nishimura, T., Mizushima, N. The ULK complex initiates autophagosome formation at phosphatidylinositol synthase-enriched ER subdomains. Autophagy 13:1795-1796 (2017) Knorr, R.L., Mizushima, N., Dimova, R. Fusion and scission of membranes: ubiquitous topological transformations in cells. Traffic 18:758-761 (2017) Galluzzi, L. et al. Molecular definitions of autophagy and related processes. EMBO J. 36:1811-1836 (2017) Koyama-Honda, I., Tsuboyama, K., *Mizushima, N. ATG conjugation-dependent degradation of the inner autophagosomal membrane is a key step for autophagosome maturation Autophagy 13:1252-1253 (2017) Mizushima, N. The exponential growth of autophagy-related research: from the humble yeast to the Nobel Prize. FEBS Lett. 591:681-689 (2017) Yoshii, S.R., Kuma, A. *Mizushima, N. Transgenic rescue of Atg5-null mice from neonatal lethality with neuron-specific expression of ATG5: Systemic analysis of adult Atg5-deficient mice. Autophagy 13:763-764 (2017) Morishita, H., Kaizuka, T., Hama, Y., *Mizushima, N. A new probe to measure autophagic flux in vitro and in vivo. Autophagy. 13:757-758 (2017) Klionsky, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12: 1-222 (2016) Noda, N.N., Mizushima, N. Atg101: not just an accessory subunit in the autophagy-initiation complex. Cell Struct. Funct. 41: 13-20 (2016) Suzuki, H., Kaizuka, T., Mizushima, N., Noda, N.N. Open and closed HORMAs regulate autophagy initiation. Autophagy. 11:2123-2124 (2015) Mizushima, N. Nbr1, a Receptor for ESCRT-Dependent Endosomal Microautophagy in Fission Yeast. Mol. Cell. 59: 887-889 (2015) Morishita, H., Mizushima, N. Autophagy in the lens. Exp. Eye Res. 144: 22-8 (2015) Jiang, P., Mizushima, N. LC3- and p62-based biochemical methods for the analysis of autophagy progression in mammalian cells Methods 75: 13-18 (2015) Yoshii, SR, Mizushima, N. Autophagy machinery in the context of mammalian mitophagy. Biochim Biophys Acta. 1853: 2797-2801 (2015) Mizushima, N. Sugar modification inhibits autophagosome-lysosome fusion. Nat. Cell Biol. 16:1132-1133 (2014) Galluzzi, L., et al. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015. Cell Death Differ. 22:58-73 (2014) Mizushima, N., Sahani, M.H. ATG8 localization in apicomplexan parasites: Apicoplast and more? Autophagy. 10:1487-1494 (2014) Shen, H.M., Mizushima, N. At the end of the autophagic road: an emerging understanding of lysosomal functions in autophagy. Trends Biochem. Sci. 39:61-71 (2014) Jiang, P., Mizushima, N. Autophagy and human diseases. Cell Res. 24:69-79 (2014) Itakura, E., Mizushima, N. Syntaxin 17: The autophagosomal SNARE. Autophagy 9:917-919 (2013) Klionsky, D.J., …. Mizushima, N., ….. et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8:445-544 (2012) Shpilka, T., Mizushima, N., Elazar, Z. Ubiquitin-like proteins and autophagy at a glance. J. Cell Sci. 125:2343-2348 (2012) Mizushima, N., Komatsu, M. Autophagy: renovation of cells and tissues. Cell 147:728-41 (2011) Klionsky, D.J., Baehrecke, E.H., Brumell, J.H., Chu, C.T., Codogno, P., Cuervo, A.M., Debnath, J., Deretic, V., Elazar, Z., Eskelinen, E.L., Finkbeiner, S., Fueyo-Margareto, J., Gewirtz, D., Jäättelä, M., Kroemer, G., Levine, B., Melia, T.J., Mizushima, N., Rubinsztein, D.C., Simonsen, A., Thorburn, A., Thumm, M., Tooze, S.A. A comprehensive glossary of autophagy-related molecules and processes (2nd edition). Autophagy 7:1273-1294 (2011) Mizushima, N. Autophagy in Protein and Organelle Turnover. Cold Spring Harb. Symp. Quant Biol. 2011 Aug 3 Mizushima, N., Yoshimori, T., Ohsumi, Y. The Role of Atg Proteins in Autophagosome Formation. Annu. Rev. Cell Dev. Biol. 27:107-32 (2011) Stappenbeck, T.S., Rioux, J.D., Mizoguchi, A., Saitoh, T., Huett, A., Darfeuille-Michaud, A., Wileman, T., Mizushima, N., Carding, S., Akira, S., Parkes, M., Xavier, R.J. Crohn disease: A current perspective on genetics, autophagy and immunity. Autophagy. 7: 1-20 (2011) Levine, B., Mizushima, N., Virgin, H.W. Autophagy in immunity and inflammation. Nature 469: 323-335 (2011) Mizushima, N. Noboru Mizushima: All about autophagy. Interview by Caitlin Sedwick. J. Cell Biol. 190: 946-947 (2010) Mizushima, N., Levine, B. Autophagy in mammalian development and differentiation. Nat. Cell Biol. 12:823-830 (2010) Klionsky, D.J., Codogno, P., Cuervo, A.M., Deretic, V., Elazar, Z., Fueyo-Margareto, J., Gewirtz, D.A., Kroemer, G., Levine, B., Mizushima, N., Rubinsztein, D.C., Thumm, M., Tooze, S.A. A comprehensive glossary of autophagy-related molecules and processes. Autophagy 2010 May 6;6(4) Kuma, A., Mizushima, N. Physiological role of autophagy as an intracellular recycling system: With an emphasis on nutrient metabolism. Semin. Cell Dev. Biol. 21: 683-90, (2010) Mizushima, N. Autophagy. FEBS Lett. 584:1279 (2010) Mizushima, N., Yoshimori, T. and Levine, B. Methods in mammalian autophagy research. Cell 140; 313-326 (2010) Mizushima, N. The role of the Atg1/ULK1 complex in autophagy regulation. Curr Opin Cell Biol. , 22:132–139 (2010) Mizushima, N. Physiological functions of autophagy. Curr Top Microbiol Immunol. 335: 71-84 (2009) Ishihara, N., Mizushima, N., A receptor for eating mitochondria. Dev. Cell 17: 1-2 (2009) Nedjic, J., Aichinger, M., Mizushima, N., Klein, L. Macroautophagy, endogenous MHC II loading and T cell selection: the benefits of breaking the rules. Curr Opin Immunol. 21: 92-97 (2009) Itakura, E., Mizushima, N., Atg14 and UVRAG: Mutually exclusive subunits of mammalian Beclin 1-PI3K complexes. Autophagy 5: 534-536 (2009) Raben, N., Baum, R., Schreiner, C., Takikita, S., Mizushima, N., Ralston, E., Plotz, P. When more is less: Excess and deficiency of autophagy coexist in skeletal muscle in Pompe disease. Autophagy 5: 111-113 (2009) Hara, T., Mizushima, N.. Role of ULK-FIP200 complex in mammalian autophagy: FIP200, a counterpart of yeast Atg17? Autophagy 5:85-87 (2009) Mizushima N. Methods for monitoring autophagy using GFP-LC3 transgenic mice. Methods Enzymol. 452:13-23 (2009) Tsukamoto, S., Kuma, A., Mizushima, N. The role of autophagy during the oocyte-to-embryo transition. Autophagy. 4: 1076-1078 (2008) Mizushima, N., Kuma, A. Autophagosomes in GFP-LC3 transgenic mice. Methods Mol. Biol. 445: 119-124 (2008) Mizushima, N., Levine, B., Cuervo, A.M., Klionsky, D.J. Autophagy fights disease through cellular self-digestion Nature 451:1069-1075 (2008) Klionsky, D.J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4: 151-75 (2008) Mizushima, N. Autophagy: process and function Genes Dev. 21: 2861-2873 (2007) Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007) Mizushima, N. Collaboration of proteolytic systems. Autophagy 3:179-180 (2007) Mizushima, N., Klionsky, D.J. Protein turnover via autophagy: Implications for metabolism. Annu. Rev. Nutr. 27:19-39 (2007) Mizushima, N. The role of mammalian autophagy in protein metabolism P. JPN Acad. B-Phys. 82: 39-46 (2007) Mizushima, N., Hara, T. Intracellular quality control by autophagy: How does autophagy prevent neurodegeneration? Autophagy 2, 302-304 (2006) Mizushima, N., A generation in autophagic vacuoles. J. Cell Biol. 171, 15-17 (2005) Mizushima, N., The pleiotropic role of autophagy: from protein metabolism to bactericide. Cell Death Differ, 12, 1535–1541 (2005) Ohsumi, Y., Mizushima, N. Tow ubiquitin-like conjugation systems essential for autophagy. Semin. Cell Dev. Biol. 15, 231-236 (2004) Mizushima, N., Methods for monitoring autophagy. Int. J. Biochem. Cell Biol. 36, 2491-2502 (2004) Mizushima, N., Yoshimori, T., Ohsumi, Y. Role of the Apg12 conjugation system in mammalian autophagy. Int. J. Biochem. Cell Biol. 35, 553-561 (2003) Mizushima, N., Ohsumi, Y., Yoshimori, T. Autohpagosome formation in mammalian cells. Cell Struct. Funct. 27, 421-429 (2002)
